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Steroid isolation , depending on context, is the isolation of chemical matter required for chemical structure elucidation, derivitzation or degradation chemistry, biological testing, and other research needs (generally milligrams to grams, but often more  or the isolation of "analytical quantities" of the substance of interest (where the focus is on identifying and quantifying the substance (for example, in biological tissue or fluid). The amount isolated depends on the analytical method, but is generally less than one microgram.  [ page needed ] The methods of isolation to achieve the two scales of product are distinct, but include extraction , precipitation, adsorption , chromatography , and crystallization . In both cases, the isolated substance is purified to chemical homogeneity; combined separation and analytical methods, such as LC-MS , are chosen to be "orthogonal"—achieving their separations based on distinct modes of interaction between substance and isolating matrix—to detect a single species in the pure sample. Structure determination refers to the methods to determine the chemical structure of an isolated pure steroid, using an evolving array of chemical and physical methods which have included NMR and small-molecule crystallography .  :10–19 Methods of analysis overlap both of the above areas, emphasizing analytical methods to determining if a steroid is present in a mixture and determining its quantity. 
Sen et al., have demonstrated that in Leishmania donovani , withaferin A inhibits PKC resulting in depolarization of Delta Psi(m) and generation of reactive oxygen species in the cell.  Depolarization leads to the release of cytochrome c and activation of caspase -like protease and DNA fragmentation, finally leading to apoptosis . Animal and in vitro studies have shown antibacterial effects against Staphylococcus aureus , Listeria monocytogenes , Bacillus anthracis , Bacillus subtilis , Salmonella enteridis and Salmonella typhimurium .  Withania roots have demonstrated chondroprotective effects in vitro arthritis model. The herb is also used in treatment of iron-deficiency anemia due to its effects on haematopoiesis and natural iron content.  Withaferin A is also used in the treatment of Metabolic syndrome .