Open Access journals are the major source of knowledge for young and aspiring generations who are keen in pursuing a career in sciences. This system provides easy access to networks of scientific journals . Authors that contribute their scholarly works to Open Access journals gain remarkable reputation as the research scholarly explore these works extensively. This process assures considerable impact factor for the journal and reputation to the authors that add value to their Academic Performance Index (API) Score. Because of the free access open access journals impact factors are improving.
Open access journal articles are essentially peer-reviewed and available for access through the directory of Open Access journals. The open access movement gained popularity after the Budapest meeting of the Open Society Institute in 2001. Under this provision, pre-prints that are yet to be reviewed can be posted online. This enables the fellow researchers of the latest updates and findings. This is provision also meant to transmit and link to the subsequent publications in the same domain.
The digital peer reviewed journals cover the novel and current scientific studies taking place across universities and research centers in various parts of the world. This leads to limitless and hassle-free dissemination of knowledge, as per the provisions of Bethesda Statement, which implies that, the transmission of digital content should be circulated among subscribers and readers without copyright restrictions. Each open access journal delivers the latest updates in the respected research area in various formats so that subscribers can access the same through various options.
With the growing number of scientific enthusiasts and readers by a large margin, the efficacy of open access publishing has witnessed an assertive impact. The importance of Peer-reviewed open access journals has also grown in modern learning environment as most of the students need a swift and instant access to published research work free of cost. Most of the open access journal articles can be cited with proper reference, which boosts the prospects of research. A quality tracking system handles the articles submitted by various authors based on quality-checking for originality and consistency for subsequent approval.
As there is a very vast scope for the research in the field of pure and Medical Sciences, various free publications are categorized into open access medical journals and open access clinical journals.
In current scenario, involving scientific research in diversified disciplines, it is necessary to publish several forms of case reports and scholarly papers. As a result, the importance of open access journals is growing significantly.
For MTT assays, cells (2,000 ~ 5,000 cells/well) were subcultured into 96-well plates according to their growth properties. Cell proliferation was assayed at 72 hr after treatment of CHIR-99021 by adding 20 μl of 5 mg/ml 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) solution per 100 μl of growth medium. After incubating for 3-4 h at 37°C, the media were removed and 150 µl/well of MTT solvent (either absolute DMSO or isopropanol containing 4 mM HCl and % Nonidet-40) was added to dissolve the formazan. The absorbance of each well was measured by ELx808 (BioTek, Winooski, VT) or Wallac Victor2 (Perkin-Elmer Life Sciences, Boston, MA) Microplate Reader. Viable cells are presented as percent of control, vehicle-treated cells.